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Literature summary for 1.4.1.9 extracted from

  • Wang, L.; Zhu, W.; Gao, Z.; Zhou, H.; Cao, F.; Jiang, M.; Li, Y.; Jia, H.; Wei, P.
    Biosynthetic L-tert-leucine using Escherichia coli co-expressing a novel NADH-dependent leucine dehydrogenase and a formate dehydrogenase (2020), Electron. J. Biotechnol., 47, 83-88 .
No PubMed abstract available
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Laceyella sacchari

Protein Variants

Protein Variants Comment Organism
synthesis production of L-tert-leucine in Escherichia coli coexpressing leucine dehydrogenase and a formate dehydrogenase. Under the optimized conditions, the double-plasmid strain transforms 1 mol/l trimethylpyruvate completely into L-tert-leucine with greater than 99.9% ee within 8 h Laceyella sacchari

Organism

Organism UniProt Comment Textmining
Laceyella sacchari
-
-
-

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Laceyella sacchari

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 half-life 12 h Laceyella sacchari

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.5
-
-
 Laceyella sacchari